The Efficient and Convenient Extracting Uranium from Water by a Uranyl-Ion Affine Microgel Container.

Uranium is an indispensable part of the nuclear industry that benefits us, but its consequent pollution of water bodies also makes a far-reaching impact on human society. The rapid, efficient and convenient extraction of uranium from water is to be a top priority. Thanks to the super hydrophilic and fast adsorption rate of microgel, it has been the ideal adsorbent in water; however, it was too difficult to recover the microgel after adsorption, which limited its practical applications. Here, we developed a uranyl-ion affine and recyclable microgel container that has not only the rapid swelling rate of microgel particles but also allows the detection of the adsorption saturation process by the naked eye.

Transcriptome analysis of grass carp (Ctenopharyngodon idella) and Holland's spinibarbel (Spinibarbus hollandi) infected with Ichthyophthirius multifiliis.

Ichthyophthirius multifiliis is a protozoan ciliate that causes white spot disease (also known as ichthyophthiriasis) in freshwater fish. Holland's spinibarbel (Spinibarbus hollandi) was less susceptible to white spot disease than grass carp (Ctenopharyngodon Idella). In this study, grass carp and Holland's spinibarbel are infected by I. multifiliis and the amount of infection is 10,000 theronts per fish. All grass carp died within 12 days after infection, and the survival rate of Holland's spinibarbel was more than 80%. In order to study the difference in sensitivity of these two fish species to I. multifiliis, transcriptome analysis was conducted using gill, skin, liver, spleen and head kidney of Holland's spinibarbel and grass carp at 48 h post-infection with I. multifiliis. A total of 489,296,696 clean reads were obtained by sequencing. A total of 105 significantly up-regulated immune-related genes were obtained by Gene Ontology (GO) classification and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis in grass carp. Cluster of differentiation 40 (CD40), cluster of differentiation 80 (CD 80), tumor necrosis factor-alpha (TNF-α), toll-like receptor 4 (TLR-4), interleukin 1 beta (IL-1ß) and other inflammatory-related genes in grass carp were enriched in the cytokine-cytokine receptor interaction pathway and toll-like receptor pathway. In Holland's spinibarbel, a total of 46 significantly up-regulated immune-related genes were obtained by GO classification and KEGG pathway enrichment analysis. Immune-related genes, such as Immunoglobin heavy chain (IgH), cathepsin S (CTSS), complement C1q A chain (C1qA), complement component 3 (C3) and complement component (C9) were enriched in phagosome pathway, lysosome pathway and complement and coagulation concatenation pathway. C3 was significantly up-regulated in gill and head kidney. Fluorescence in situ hybridization (FISH) showed that the C3 gene was highly expressed in gill tissue of Holland's spinibarbel infected with I. multifiliis. A small amount of C3 gene was expressed in the gill arch of grass carp after infected with I. multifiliis. In conclusion, the severe inflammatory response in vivo after infecting grass carp with I. multifiliis might be the main cause of the death of grass carp. The extrahepatic expression of the gene of Holland's spinibarbel might play an important role in the immune defense against I. multifiliis.

Assessing individual environmental exposure derived from the spatiotemporal behavior context and its impacts on mental health.

Research on the associations between environmental exposures and mental health has attracted considerable attention. Most studies to date have mainly estimated environmental health effects based on static geographic contexts (e.g., residential neighborhoods, administrative units), ignoring the dynamic nature of individual spatiotemporal behavior, which may lead to unreliable results. To address this limitation, this study collects survey data from 1003 adults in Guangzhou, China. Then, it delineates dynamic geographic context to capture individual daily activity and travel and assesses individual exposure to environmental factors derived from the home buffer (HB) and the time-weighted activity and travel buffer (TATB). Finally, multiple linear regression models are used in this paper to examine and compare the relationships between individual environmental exposure and mental health based on the HB and TATB. The results of this study indicate that there are great differences in individual environmental exposure levels based on the HB and TATB. The explanatory power of the environmental factors obtained from the TATB on mental health is greater than that derived from the HB. Specifically, exposures to some environmental factors (i.e., green space coverage, blue space coverage, fitness facility density, and recreational facility density) derived from the TATB have mental health-promoting effects, while exposures to the other environmental factors (i.e., public transit station density) have mental health-constraining effects. These findings enrich our knowledge of spatiotemporal behavior and the effects of the dynamic contextual environment on mental health, as well as provide valuable implications for urban planning and public health service.

Organoarsenic conversion to As(III) in subcritical hydrothermal reaction of livestock manure.

Liquid phase produced by the subcritical hydrothermal liquefaction (HTL) of livestock manure is extensively used in agronomic and environmental applications, but the potential risks caused by inherent pollutants (e.g., roxarsone, ROX) of the livestock manure have not been considered. This study shows that less toxic ROX is completely converted into highly toxic As(III) and As(V) in the HTL reaction with temperature more than 240 °C. Moreover, more than 81.5% of As is distributed in the liquid phase generated by the livestock manure HTL reaction. Notably, the hydrothermal products of livestock manure facilitate the conversion of As(V) to As(III). The resulting hydrochar and aldehydes act as electron donors for As(V) reduction, thus resulting in the formation of As(III). Furthermore, the dissociated As promotes the depolymerization and deoxygenation of the macromolecular compounds to produce more small oxygen-containing compounds such as aldehydes, further boosting the As(V) reduction to As(III). These results indicate that the liquid phase of the livestock manure has potential risks in applications as a fertilizer. Such findings have substantial implications in biomass utilization and redox reactions of envirotechnical and biogeochemical relevance.

Extraneous Fe Increased the Carbon Retention of Sludge-Based Biochar.

Pyrolysis is a promising technology for the disposal of sewage sludge. In this study, FeCl3 was selected as an additive for sludge pyrolysis. The results indicated that the FeCl3-addition strategy not only enhanced carbon retention but also enhanced the nitrogen retention of sludge-based biochar. The best enhancement effect both occurred at 500°C, and the enhanced amount reached 29.7 mg C/g sludge and 1.33 mg N/g sludge, respectively. This enhancement may be attributed to the physical isolation provided by newly formed metallic complexes or oxides. Besides, the added FeCl3 improved the polarity and aromaticity of modified biochar by retaining more oxygen-containing functional groups, and could also catalyze the decomposition of tar, resulting in the release of more small molecular substances. The quantitative estimation of carbon and nitrogen retention in provinces of China found that the enhancement in coastal provinces was significantly preceded that of inland provinces.

Molecular characterization and environmental impacts of water-soluble organic compounds of bio-oil from the thermochemical treatment of domestic sewage sludge.

Water-soluble organic compounds derived from bio-oil (WOCB) are regarded as potential risk sources of sludge thermochemical treatment. This study showed that 10.35 mg of water-soluble organic carbon and 1.32 mg of water-soluble organic nitrogen were released per gram of sludge when the final temperature of thermochemical treatment was 600 °C. WOCB was mainly formed at 300-500 °C. Furthermore, FT-ICR MS results indicated that high temperatures promoted deamination reactions, and low molecular weight (LMW) compounds with low oxygen number polymerized into aromatic compounds with increasing temperature. Noteworthily, WOCB released at 20-600 °C showed strong phytotoxicity to wheat. LMW compounds with lignin/carboxylic rich alicyclic molecules (CRAM)-like structures derived from low temperatures (200-400 °C) induced this inhibitory effect, but lipids containing nitrogen and sulfur from high temperatures (400-600 °C) can act as nutrients to promote wheat growth. This study provides theoretical support for the risk control and benefits assessments of sludge thermochemical treatment.

Reveal a hidden highly toxic substance in biochar to support its effective elimination strategy.

With the aim to develop optimized biochar with minimal contaminants, it is important significance to broaden the understanding of biochar. Here, we disclose for the first time, a highly toxic substance (metal cyanide, MCN, such as KCN or NaCN) in biochar. The cyanide ion (CN-) content in biochar can be up to 85,870 mg/kg, which is determined by the inherent metal content and type in the biomass with K and Na increasing and Ca, Mg and Fe decreasing its formation. Density functional theory (DFT) analysis shows that unstable alkali oxygen-containing metal salts such as K2CO3 can induce an N rearrangement reaction to produce for example, KOCN. The strong reducing character of the carbon matrix further converts KOCN to KCN, thus resulting biochar with high risk. However, the stable Mg, Ca and Fe salts in biomass cannot induce an N rearrangement reaction due to their high binding energies. We therefore propose that high valent metal chloride salts such as FeCl3 and MgCl2 could be used to inhibit the production of cyanide via metal interactive reaction. These findings open a new point of view on the potential risk of biochar and provide a mitigation solution for biochar's sustainable application.

Reduction of serum cholesterol and its mechanism by Lactobacillus plantarum H6 screened from local fermented food products.

Hypercholesterolemia is the leading cause of cardiovascular disease worldwide. In this study, the lactic acid bacteria (Lactobacillus plantarum H6) screened from homemade fermented foods in northeastern China were used to study their cholesterol-lowering ability and to analyze their degradation mechanism. Results showed that L. plantarum H6 significantly reduced serum cholesterol levels in C57BL/6 mice fed a hypercholesterolemia diet. It mainly promoted the expression of CYP7A1 gene by inhibiting the farnesoid X receptor pathway to increase the in vivo synthesis of bile acids and improved the intestinal microbial community structure of C57BL/6 mice to increase the abundance of bacterial flora containing bile salt hydrolase activity. Hence, L. plantarum H6 played a role in reducing the cholesterol content in mice.

Linoleic acid inhibits Lactobacillus activity by destroying cell membrane and affecting normal metabolism.

BACKGROUND: The reason why dietary polyunsaturated fatty acids (PUFAs) affect the activity of Lactobacillus remains unclear. In this study, linoleic acid was used to study the mechanism underlying its inhibition function against Lactobacillus activity. RESULTS: The growth curve of Lactobacillus rhamnosus LGG and the metabolite content in bacterial liquid were determined at varying linoleic acid concentration. The degree of cell membrane damage of L. rhamnosus LGG was determined by flow cytometry and fluorescence microscopy, and the cell structure was observed by scanning electron microscopy and transmission electron microscopy. The effect of linoleic acid on Lactobacillus activity was assessed in a simulated gut environment. Results showed that L. rhamnosus LGG grew slowly, cell metabolites leaked into the liquid, cell membrane was damaged, and the cell structure changed at a linoleic acid concentration of 50 µg mL-1 . CONCLUSION: The mechanism of action of linoleic acid on Lactobacillus showed that that linoleic acid destroyed the cell membrane of bacteria, thereby affecting the normal metabolism of the bacteria and ultimately leading to their death. © 2019 Society of Chemical Industry.

Conversion of phosphorus and nitrogen in lincomycin residue during microwave-assisted hydrothermal liquefaction and its application for Pb2+ removal.

Treatment of antibiotic fermentative residue (AFR) produced from pharmaceutical industries and their application in the environment has been gaining researchers' interest. In this study, lincomycin residue (LMR, the type of AFR) was treated with microwave-assisted hydrothermal liquefaction (MW-HTL) in a temperature range 120-210 °C, transforming effect of phosphorus (P) and nitrogen (N) functional groups in LMR samples was characterized with elemental analysis, XRD, XPS, FT-IR, and P-extraction, and utilized LMR samples for Pb2+ removal from aqueous solutions. The temperature had a significant impact on P and N functional groups conversion justified by characterization techniques and also responsible for Pb2+ adsorption. LMR hydrochar produced at 210 °C was accounted highest Pb2+ adsorption capacity (57.4 mg g-1), higher four folds than raw LMR (13.8 mg g-1). To understand the mechanism and rate defining phase of adsorption equilibrium isotherm and kinetic models were applied systematically. Adsorption results of LMR and its derived hydrochar samples found connectivity with Langmuir and pseudo-first-order isotherm models. Adsorption mainly occurred as ion-exchange dependent on the substitution of metal ions (Pb2+) to Ca2+ ions present in P-materials, and surface adsorption dependent on surface functional groups of LMR samples. Better operation feasibility of MW-HTL treated LMR, elaboration of P and N conversion behavior and high sorption of Pb2+ ions could make LMR a frontrunner for heavy metals immobilization.

Associations between vitamin D receptor genetic variants and tuberculosis: a meta-analysis.

We performed a meta-analysis to evaluate potential associations between vitamin D receptor ( VDR) genetic variants and tuberculosis (TB). Systematic literature research was conducted in PubMed, Web of Science, and Embase. We calculated odds ratios (ORs) and 95% confidence intervals (CIs) to estimate strength of associations in all possible genetic models, and P values ≤ 0.05 were considered to be statistically significant. In total, 42 studies were enrolled for analyses. Pooled overall analyses suggested that VDR rs1544410 (dominant model: P = 0.02; allele model: P = 0.03) and rs731236 (dominant model: P = 0.04; recessive model: P = 0.02; allele model: P = 0.01) variants were significantly associated with TB. Further subgroup analyses by ethnicity revealed that rs1544410 (dominant and allele models) and rs731236 (dominant, recessive, and allele models) variants were both significantly associated with TB in South Asians. When we stratified data by type of disease, positive results were detected for rs7975232 variant in EPTB (dominant, recessive, over-dominant, and allele models) subgroup, and for rs2228570 variant in PTB (dominant, recessive, and allele models) and EPTB (dominant, recessive, over-dominant, and allele models) subgroups. Our meta-analysis supported that rs7975232, rs1544410, rs2228570, and rs731236 variants might serve as genetic biomarkers of certain types of TB.

In vivo assessment of immunomodulatory activity of hydrolysed peptides from Corylus heterophylla Fisch.

BACKGROUND: Hazelnut dregs are by-products of hazelnut oil expression, which have not been fully exploited. This research aims to assess the immunomodulatory function of hazelnut hydrolysed peptides (HHPs). RESULTS: HHPs with a hydrolysis degree of 38.08% were divided into three fractions by ultra-filtration: the high molecular weight peptide (>10 kDa), medium molecular weight peptide (3 kDa to 10 kDa), and low molecular weight peptide (<3 kDa). Mice were fed daily with HHPs of different molecular weights at doses of 200, 400, and 800 mg kg(-1) body weight. On the 10th, 20th and 30th day of feeding, representative immune indexes were measured. Results showed that HHPs can regulate the immune system of mice, which is affected by the molecular weight of HHP and the feeding time. Generally, short-term feeding (10 d to 20 d) with HHPs of different molecular weights can improve most immune indexes (organ index, spleen lymphocyte proliferation, macrophage activity, secretory immunoglobulin A content, and number of CD4(+) and CD8(+) T cells), whereas during long-term feeding (30 d), low molecular weight HHP can better sustain immune regulation. CONCLUSION: HHPs exhibit potential immunomodulatory properties, which has promising implications for the development of new functional foods. © 2015 Society of Chemical Industry.

[Evaluation of dietary pattern and nutritional status of residents in southeast coastal area].

OBJECTIVE: To evaluate the dietary pattern and nutritional status of urban residents in southeast coastal area. METHODS: A dietary survey concerning 1332 persons aged 18 and over was carried out with dietary inquiry and 24-hour recall methods from August to December in 2009. RESULTS: The intakes of cereal, meat, eggs, seafood were enough. The consumption of milk and dairy products, vegetables, fruits was insufficient while the amount of oil was too high. Among them, the intake of milk and dairy products was only 1/3 of suggested values in Dietary Guideline and Balanced Diet Pagoda for Chinese Residents. The intakes of protein, retinol, iron and selenium were sufficient, while those of calcium, thiamine, riboflavin, ascorbic acid were too less than the dietary reference intakes (DRIs). CONCLUSION: The dietary pattern of urban residents in Ningbo was not reasonable. Nutrition education should be strengthened to guide residents for planning reasonable and balanced diets.

Identification of cytochrome P450 monooxygenase genes from the white-rot fungus Phlebia brevispora.

Three cytochrome P450 monooxygenase (CYP) genes, designated pb-1, pb-2 and pb-3, were isolated from the white-rot fungus, Phlebia brevispora, using reverse transcription PCR with degenerate primers constructed based on the consensus amino acid sequence of eukaryotic CYPs in the O2-binding, meander and heme-binding regions. Individual full-length CYP cDNAs were cloned and sequenced, and the relative nucleotide sequence similarity of pb-1 (1788 bp), pb-2 (1881 bp) and pb-3 (1791 bp) was more than 58%. Alignment of the deduced amino acid (aa) sequences of pb-1-pb-3 showed that these three CYPs belong to the same family with > 40% aa sequence similarity, and pb-1 and pb-3 are in the same subfamily, with > 55% aa sequence similarity. Furthermore, pb-1-pb-3 appeared to be a subfamily of CYP63A (CYP63A1-CYP63A4), found in Phanerochaete chrysosporium. The phylogenetic tree constructed by 500 bootstrap replications using the neighbor-joining method showed that the evolutionary distance between pb-1 and pb-3 was shorter than that between pb-2 and pb-1 (or pb-3). Exon-intron analysis of pb-1 and pb-3 showed that both genes have nearly the same number, size and order of exons and the types of introns, also indicating both genes appear to be evolutionarily close. It is interesting that the transcription level of pb-3 was evidently increased above the pb-1 transcription level by exposure to 12 coplanar PCB congeners and 2,3,7,8-tetrachlorodibenzo-p-dioxin, though the two genes were evolutionarily close.

[Analysis of mood factors in prostatitis and Molida therapy].

OBJECTIVES: To analyze the mood factors in prostatitis and evaluate the effects of Molida psychological therapy. METHODS: Two hundred and thirty-six chronic prostatitis patients were divided according to the course of disease into above 6 months group (n = 31) and below 6 months group(n = 205) as well as into sexual disease group(n = 25) and non-sexual disease group(n = 211). An investigation was made by self-rating method on the basis of the National Institutes of Health Chronic Prostatitis Symptom Index(NIH-CPSI) and Symptom Checklist 90 (SCL-90) and 56 cases were rated again after Molida therapy. RESULTS: 1. The scores of SCL-90 in 236 prostatitis patients were significantly higher than normal(P < 0.01). The factor scores of SCL-90 showed one-item positive in 107 patients (45.2%), of whom 27 (25.2%) had depressive disorder and 80(74.77%) had anxiety (23 with significant compulsion). Thirty-eight cases(16.1%) were two items positive. 2. The scores of SCL-90 were significantly higher in the > 6 months group of history and the sexual disease group than in the control group (P < 0.01). 3. The scores of NIH-CPSI showed a positive correlation with those of SCL-90 and both scores in 43/56 cases were significantly decreased after psychological treatment (P < 0.01). CONCLUSIONS: The mood factor plays an important role in aggravating symptoms in chronic prostatis patients and causes difficulty for management. Molida may significantly improve the mood and symptoms of the chronic prostatitis patient.